Warwickshire Wildlife Trust is demanding a public inquiry over the proposal to develop a complementary technology park alongside Jaguar's hi-tech design centre.
Coventry City Council believes the project could create 2,500 jobs and is anxious to accommodate it.
Carl Pearson, head of the city's centre for investment, said: 'Ultimately this is about the development of the global motor industry and ensuring that Coventry has a place in it. It's important that we are seen as a centre of excellence with world-class facilities.'
He said: 'We're not anti-development but we are pro-nature.'
Jaguar does not want to develop an alternative brownfield site because it is not immediately adjacent to their centre.